The technique that was developed by Hogeboom and Schneider was really the same as Claude's but adapted to the new medium, sucrose, but, essentially, it involved separation of four fractions: nuclear fraction; the mitochondrial fraction; the microsomal fraction; and the supernatant. And when we looked at the results of our own fractionation results, with acid phosphatase and later with beta glucuronidase, we were puzzled or embarrassed by the fact that only about two thirds of the activity of those enzymes was recovered with the mitochondria. And almost one third came down with the microsomes. Now that finding had three possible explanations. You see the Jesuit logic coming back; it's the only thing that helped me in research because I wasn't very good with my hands and I wasn't very good with instruments or techniques, and many of my co-workers were very helpful in this respect. I loved using my hands but I wasn't very skilled.