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Views | Duration | ||
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61. Solving the structure of a two-zinc finger construct | 77 | 06:53 | |
62. Repertoire selection technology | 70 | 04:21 | |
63. Phage display and solving the 'mystery' of the stereochemical code | 63 | 05:57 | |
64. Refining the structure of zinc fingers | 58 | 03:10 | |
65. Zinc finger binding | 95 | 01:03 | |
66. Intervening in gene expression for the first time | 70 | 07:14 | |
67. Trying to improve the zinc finger constructs | 74 | 03:09 | |
68. Experimenting with zinc finger constructs | 69 | 03:15 | |
69. Yen Choo's company: Gendaq | 494 | 03:25 | |
70. Making zinc finger archives | 88 | 02:52 |
We also by the way showed that the linker, Yen Choo showed this, that the linker DNA once the finger's bound to the DNA, the linker, the five amino acids also folded up in a pretty great way and bound the sequence of DNA, the contributor of the binding. And we found that with three fingers, binding to nine base pairs you could get nano-molar binding. Which was pretty good for three fingers. One finger on its own binds very weakly, it... they're synergistic you see so you don't get one finger binds barely with... micro-molar binding or something like that.
[Q] So the... the specificity is higher...
Three fingers, well yes, but it’s not all that high because there are many different sequences it will bind to so the question was how do you get high specificity and high affinity.
Born in Lithuania, Aaron Klug (1926-2018) was a British chemist and biophysicist. He was awarded the Nobel Prize in Chemistry in 1982 for developments in electron microscopy and his work on complexes of nucleic acids and proteins. He studied crystallography at the University of Cape Town before moving to England, completing his doctorate in 1953 at Trinity College, Cambridge. In 1981, he was awarded the Louisa Gross Horwitz Prize from Columbia University. His long and influential career led to a knighthood in 1988. He was also elected President of the Royal Society, and served there from 1995-2000.
Title: Zinc finger binding
Listeners: John Finch Ken Holmes
John Finch is a retired member of staff of the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK. He began research as a PhD student of Rosalind Franklin's at Birkbeck College, London in 1955 studying the structure of small viruses by x-ray diffraction. He came to Cambridge as part of Aaron Klug's team in 1962 and has continued with the structural study of viruses and other nucleoproteins such as chromatin, using both x-rays and electron microscopy.
Kenneth Holmes was born in London in 1934 and attended schools in Chiswick. He obtained his BA at St Johns College, Cambridge. He obtained his PhD at Birkbeck College, London working on the structure of tobacco mosaic virus with Rosalind Franklin and Aaron Klug. After a post-doc at Childrens' Hospital, Boston, where he started to work on muscle structure, he joined to the newly opened Laboratory of Molecular Biology in Cambridge where he stayed for six years. He worked with Aaron Klug on virus structure and with Hugh Huxley on muscle. He then moved to Heidelberg to open the Department of Biophysics at the Max Planck Institute for Medical Research where he remained as director until his retirement. During this time he completed the structure of tobacco mosaic virus and solved the structures of a number of protein molecules including the structure of the muscle protein actin and the actin filament. Recently he has worked on the molecular mechanism of muscle contraction. He also initiated the use of synchrotron radiation as a source for X-ray diffraction and founded the EMBL outstation at DESY Hamburg. He was elected to the Royal Society in 1981 and is a member of a number of scientific academies.
Tags: Yen Choo
Duration: 1 minute, 4 seconds
Date story recorded: July 2005
Date story went live: 24 January 2008